Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Meridian Life Science Inhibitor-Tolerant qPCR Mix, 5ML
Meridian’s universal Inhibitor-Tolerant qPCR is a 2x formulation ready-to-use quantitative PCR supermix, containing all components except primers, probes and templates for direct qPCR. It is designed for developing qPCR multiplex assays that require minimal sample processing and fast turn-around times (TAT). Because the mix is highly resistant to various qPCR inhibitors, it can be used with crude lysates or inhibitor-rich samples to directly detect pathogens, including viruses and bacteria, in various biological specimens and to analyze gene expression in cells.
Alkali Scientific Radiant 1-Step SYBR Green Lo-ROX qPCR Kit, 1200 Reactions, (12 x 1mL 2X Green 1-Step LO-ROX Mix, 12 x 0.2mL 20X RTase for qPCR 1-Step)
Radiant™ 1-Step SYBR Green Kit uses the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The qPCR is performed by Radiant™ Taq HS DNA Polymerase, resulting in robust RT-PCR performance.Radiant™ 1-Step SYBR Green Kit can be used to quantify any RNA template including mRNA, total RNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. Proprietary small molecular inhibitor technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced buffer chemistry, we offer market leading performance with minimal or no optimization.Many modern real-time PCR instruments have a fast cycling mode.
As One International Inc RealQ Plus 2x Master Mix for Probe high ROX 4000R, 4000Rxn
Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of SNP or quantitative gene transcripts. AS ONE offersthe Real Q-PCR Master Mix, a single-tube 2X reagent ideal for most Quantitative PCR applications. The Real Q-PCR kit has been designed for optimal performance on most of the commonly used Real time PCR instruments. The Real Q-PCR kits includes the components necessary for performing DNA amplification, and have been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA and plasmid DNA. The Real Q-PCR master mix includes the HS DNA polymerase, a modified Taq DNA polymerase with hot start capabilities. The HS enzyme improves the PCR amplification reaction by decreasing background from non-specific amplification and increases amplification of desired products.
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR 4X Mix with UDG - 500 rxns
The Luna Probe One-Step RT-qPCR 4X Mix with UDG is designed for real-time detection of target RNA sequences using hydrolysis probes. In a single tube, RNA is first converted to cDNA by a reverse transcriptase, then a DNA-dependent DNA polymerase amplifies the cDNA, enabling quantitation via real time or quantitative PCR (qPCR). Probe-based qPCR/RT-qPCR monitors an increase in fluorescence upon 5' to 3' exonuclease cleavage of a quenched, target-specific probe to measure DNA amplification at each cycle of a PCR. At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle or Cq value can be determined. Cq values can be used to evaluate relative target abundance between two or more samples.
Meridian Life Science qPCR Extraction Control Red, 10ML
Molecular IVD companies continue to experience tighter and tighter regulations in regard to incorporating appropriate positive and negative controls into their assays. Meridian’s qPCR Extraction Control Red uses E. coli cells of a known concentration that contain quantitative PCR positive control DNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample target DNA). The cells are added to the sample and lysed and extracted with the sample DNA for downstream amplification by qPCR. The detection of qPCR Extraction Control acts as a PCR positive control and confirms the success of the extraction and amplification steps, avoiding the misinterpretation of a false negative result.
ACROBiosystems E1A&SV40LTA resDNA Quantitation Kit (qPCR)
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
Meridian Life Science Inhibitor-Tolerant qPCR Mix 5x, 40ML
Meridian’s universal Inhibitor-Tolerant qPCR is a 5x formulation ready-to-use quantitative PCR supermix, containing all components except primers, probes and templates for direct qPCR. It is designed for developing qPCR multiplex assays that requiremore room for sample, minimal sample processing and fast turn-around times (TAT). Because the mix is highly resistant to various qPCR inhibitors, it can be used with crude lysates or inhibitor-rich samples to directly detect pathogens, including viruses and bacteria, in various biological specimens and to analyze gene expression in cells.
Zymo Research Corporation Quick-cfRNA Serum & Plasma Kit
The Quick-cfRNA Serum & Plasma Kit enables simple, reliable, and efficient preparation of high-quality circulating cell-free RNA (including protein-bound, exosomal, miRNA and other small RNAs) from serum, plasma and other biological fluids. The kit does not use toxic phenol-chloroform or cumbersome protein precipitation and removal steps. Recovered cell-free RNA scales linearly relative to the sample input volume up to 3 ml. ZymoSpin™ Technology enables isolation of ultra-pure cell-free RNA suitable for all subsequent analyses, such as RT-qPCR and NGS. Empower your discovery of circulating RNA biomarkers.
Meridian Life Science RT-qPCR Extraction Control Red, 1ML
Molecular IVD companies continue to experience tighter and tighter regulations in regard to incorporating appropriate positive and negative controls into their assays. Meridian’s RT-qPCR Extraction Control Red uses artificial micelles that contain quantitative PCR positive control RNA sequence (with no known homology to any organism, so that it does not interfere with the detection of the sample target RNA). The cells are added to the RT-qPCR Extraction Control is spiked in the with lysis buffer into the sample prior to RNA extraction for downstream amplification by RT-qPCR. The detection of RT-qPCR Extraction Control acts as a cDNA synthesis and PCR positive control and confirms the success of the extraction and amplification steps, avoiding the misinterpretation of a false negative result.